Succeed escape: Flow shear promotes tumbling of Escherichia colinear a solid surface

نویسندگان

  • Mehdi Molaei
  • Jian Sheng
چکیده

Understanding how bacteria move close to a surface under various stimuli is crucial for a broad range of microbial processes including biofilm formation, bacterial transport and migration. While prior studies focus on interactions between single stimulus and bacterial suspension, we emphasize on compounding effects of flow shear and solid surfaces on bacterial motility, especially reorientation and tumble. We have applied microfluidics and digital holographic microscopy to capture a large number (>105) of 3D Escherichia coli trajectories near a surface under various flow shear. We find that near-surface flow shear promotes cell reorientation and mitigates the tumble suppression and re-orientation confinement found in a quiescent flow, and consequently enhances surface normal bacterial dispersion. Conditional sampling suggests that two complimentary hydrodynamic mechanisms, Jeffrey Orbit and shear-induced flagella unbundling, are responsible for the enhancement in bacterial tumble motility. These findings imply that flow shear may mitigate cell trapping and prevent biofilm initiation.

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Corrigendum: Succeed escape: Flow shear promotes tumbling of Escherichia coli near a solid surface

“This research was made possible in part by a grant from The Gulf of Mexico Research Initiative (Grant No. SA12-03/GoMRI-003), and in part by grants from NSF (Grant No. CBET-1341901) and NIH (Grant No. 1-R21-EB008844-01). Data are publicly available through the Gulf of Mexico Research Initiative Information & Data Cooperative (GRIIDC) at https://data.gulfresearchinitiative.org (doi:10.7266/N744...

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عنوان ژورنال:

دوره 6  شماره 

صفحات  -

تاریخ انتشار 2016